High-throughput mapping of regulatory DNA
نویسندگان
چکیده
منابع مشابه
High-throughput methods of regulatory element discovery.
With the number of organisms whose genomes have been sequenced, a vast amount of information concerning the genetic structure of an organism's genome has been collected. However, effective experiment means to study how this information is accessed have only recently been developed. In this review, three basic methods for identifying regions of protein-DNA interaction will be introduced. The fir...
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MOTIVATION A ubiquitous and fundamental step in high-throughput sequencing analysis is the alignment (mapping) of the generated reads to a reference sequence. To accomplish this task, numerous software tools have been proposed. Determining the mappers that are most suitable for a specific application is not trivial. RESULTS This survey focuses on classifying mappers through a wide number of c...
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BACKGROUND The accuracy and precision of estimates of DNA concentration are critical factors for efficient use of DNA samples in high-throughput genotype and sequence analyses. We evaluated the performance of spectrophotometric (OD) DNA quantification, and compared it to two fluorometric quantification methods, the PicoGreen assay (PG), and a novel real-time quantitative genomic PCR assay (QG) ...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2016
ISSN: 1087-0156,1546-1696
DOI: 10.1038/nbt.3468